The first DNA sequencing method was developed by Frederick Sanger in 1977. The technique was based on incorporation of chain-terminating dideoxynucleotides by DNA polymerase while replicating DNA. So ...
Sanger sequencing is a DNA sequencing method developed by Fred Sanger in 1977. The technique is based on incorporation of chain-terminating dideoxynucleotides by DNA polymerase during replication of ...
32 P-label is introduced by incoming dGT 32 P molecule. The arrows indicate the sites of enzymatic hydrolysis. The bases of the template DNA strand are designated as clear boxes, and the bases in the ...
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