We next examined whether a 5′-dRP/AP lyase was also important in NHEJ of such substrates in cells. We used mouse dermal fibroblasts 13 deficient in Ku70 and p53, either complemented by expression of a ...
Once the rHSV co-infection parameters (MOI ratio, cell density, harvest timing) were established for HEK293 cells, the method was applied to other cell types to explore the robustness of the ...