Within multi-exon genes, after introns are removed, the exons must be spliced back together. Splice-enhancer domains (exon sequences near the intron–exon boundary) help to ensure that this happens ...

The polypyrimidine tract binding protein (PTB) binds pre-mRNAs to alter splice-site choice. We characterized a series of spliceosomal complexes that assemble on a pre-mRNA under conditions of either ...

One of the most long-standing, fundamental mysteries of biology surrounds the poorly understood origins of introns. Introns are segments of noncoding DNA that must be removed from the genetic code ...

This is a preview. Log in through your library . Abstract RNA is commonly believed to undergo a number of sequential folding steps before reaching its functional fold, i.e., the global minimum in the ...

During RNA maturation, the group I intron promotes two sequential phosphorotransfer reactions resulting in exon ligation and intron release. Here, we report the crystal structure of the intron in ...