Adapters consist of a core sequence (black) and an enzyme-specific sequence (red). The enzyme-specific sequence allows the ligation of the adapters to the resulting restriction fragments (green) ...
Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...
Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...
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