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Protein concentrations are tightly controlled in pure protein assays. Completing a Bradford standard curve is a tool I use to monitor my protein concentrations. After completing a standard curve, I take absorbance readings of my pure protein samples, do some math, and figure out what my protein concentrations are! From there I typically have to dilute my proteins to get the concentrations needed for my specific assays. The pipetting never stops👩‍🔬🫣🧬 #womeninstem #phd #science #research #lab
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Karmen: The assay uses a coupled enzymatic reaction with malate dehydrogenase (MDH) and NADH. It measures the decrease in absorbance at 340 nm as NADH is oxidized to NAD . To quantify AST activity in blood serum, indicating liver function or damage. #medbeks #rmt #medtechstudent #medtech #medtechlife
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absorbance = molar absorptivity times length of light path times concentration 🥰 🎥 uta bc this lab isnt in our curriculum 💀 #chemtok #chemistry #chemistrylab #chemistryexperiment #womeninstem #genchem #chemistrymemes #chemlab #fypシ
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